Purification and Characterization of Heparinase from FZauobacterium heparinurn"
نویسنده
چکیده
From the "Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, the 'College of Pharmacy, University of Iowa, Division of Medicinal Chemistry, Iowa City, Iowa 52242, the 'Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, the dWhitaker College of Health Sciences, Management and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, and the 'Department of Surgery, Children's Hospital Medical Center, Boston, Massachusetts 02115
منابع مشابه
Purification and Characterization of Heparin Lyases from Flavobacterium heparinurn”
Heparin lyase I has been purified from Flavobacterium heparinum and has been partially characterized (Yang, V. C., Linhardt, R. J., Berstein, H., Cooney, C. L., and Langer, R. (1985) J. Biol. Chem. 260, 18491857). There has been no report of the purification of the other polysaccharide lyases from this organism. Although all three of these heparinlheparan sulfate lyases are widely used, with th...
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The use of heparin for extracorporeal therapies has been problematical due to haemorrhagic complications; as a consequence, heparinase I from Flavobacterium heparinum is used for the determination of plasma heparin and for elimination of heparin from circulation. Here we report the expression of recombinant heparinase I in Escherichia coli, purification to homogeneity and characterization of th...
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A unique heparinase was isolated from a recently discovered Gram-negative soil bacterium. The enzyme (heparinase III) was purified by hydroxylapatite chromatography, chromatofocusing, and gel permeation chromatography. The enrichment was 48x, and the specific activity of catalytically pure heparinase was 127 IU/mg of protein. Similar to the heparinase I from Flavobacterium heparinum, heparinase...
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Crude enzyme obtained from heparin-induced flavobacteria has been fractionated into a heparitinase acting on heparitin sulfates and related compounds and a heparinase acting mainly on heparin. Purification achieved for each was from 50 to 100 times that of earlier preparations containing a mixture of the two enzymes. In agreement with previous data both enzymes act as eliminases rather than hyd...
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تاریخ انتشار 2001